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. 2023 Oct 9;14:6322. doi: 10.1038/s41467-023-41891-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Experimental outline: primary murine microglia were purified and exposed to LPS for a time course of 30 min to 24 h before lysis, TMT-multiplexing and proteomic and phosphoproteomic analysis. b Quantification of significantly up- and downregulated proteins in proteomic analysis of microglia treated with LPS. For full table see Supplementary Data 1. c, d Gene ontology analysis of significantly upregulated proteins for cellular component and biological process after 8 h LPS treatment. For full table see Supplementary Data 2. e Volcano plots of significantly down- and upregulated proteins at indicated timepoints highlighting proteins associated with “Inflammatory response” (green) and selected inflammatory markers (purple). Non-significantly regulated proteins are omitted. GO: Gene oncology. f Volcano plot as in e, but highlighting proteins associated with “Microtubules” or “Microtubule organizing centers” (green) and selected examples (orange). g Number of significantly (de-) phosphorylated proteins after LPS treatment at indicated timepoints. For full table see Supplementary Data 1. h, i Gene ontology analysis of significantly upregulated peptides for cellular component and biological process in the 8 h LPS treatment. For full table see Supplementary Data 2. j Volcano plot of de-phosphorylated and phosphorylated peptides after 0.5 h LPS treatment highlighting proteins associated with “Microtubules” (blue) or “Microtubule organizing centers” (green). Non-significantly regulated peptides are omitted. k Volcano plot of de-phosphorylated and phosphorylated peptides at indicated timepoints highlighting phosphorylated peptides of Map4 (red) and Stmn1 (teal). Non-significant peptides are omitted. Source data are provided as a Source Data file.