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. 2023 Oct 9;13:16994. doi: 10.1038/s41598-023-43435-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Elution efficiency assessment of seqIF. (a) Elution efficiency and epitope stability of CD20 staining on a human tonsil FFPE sample across 20 elution cycles. Scale bar: 50 μm. (b) Example of elution followed by negative controls on different tissue samples, with elution efficiency indicated as a percentage. The first panel shows the elution efficiency of the markers CD45, CD68, and CD19 on a human FFPE tonsil sample. The second panel shows the elution efficiency of the markers E-Cadherin, CD3, and MYL9 on a human FFPE colorectal cancer sample. The third panel displays the elution efficiency of CD45, GFAP, and F4/80 on different mouse frozen section tissues (spleen, brain, and liver). Scale bars: 50 μm.