Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Oct 9;13:16994. doi: 10.1038/s41598-023-43435-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Demonstration of aspects that ensure image quality with seqIF. (a) Quantification of the horizontal and vertical signal variation of a staining for CD20 on human tonsil FFPE performed and imaged on COMET™. The normalized signal intensities of 25 regions of interest (left, blue squares) were plotted according to their horizontal (right graph, blue) or vertical position (right graph, orange). Overall signal variation for both axes was determined to be below 6%. Both X and Y values are represented in millimeters. Scale bar: 1 mm. (b) Example of the dynamic range of a FoxP3 seqIF staining (left) and pseudo coloring with a Fire look up table (right) highlighting low, mid, and high expressing cells. The scale represents grey values of the 16-bit images (1 to 65,535). (c) Automated flat-field correction provides uniform seqIF images. The result is a seamless mosaic of the whole image without aberrations (right) compared to an image without correction and visible aberrations (left).