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. 2023 Sep 26;17:1277268. doi: 10.3389/fncel.2023.1277268

Figure 2.

Figure 2

Immunofluorescence analysis of NLRP1 inflammasome at 6-h, 24-h, or 72-h following I/R. (A) NLRP1 inflammasome protein was detected with immunofluorescence staining (n = 5 mice/group). Images of Hoechst-33258-labeled cell nuclei (blue) were overlapped with the images of NLRP1 (40X, scale bar = 25 μm). NLRP1 inflammasome complex, whose expression was increased in the cell cytoplasm 6, 24, or 72-h after I/R, was decreased by 12/15-LOX inhibition (ML351). In the ML351 treated group at 24-h after I/R, NLRP1 appears to be expressed in both the soma and its axonal extension, which may indicate that it is neuronal (White arrow). (B) Quantitative analysis showed that NLRP1 increased in DMSO groups at 6-h (**p = 0.002), 24-h (****p < 0.0001), and 72-h (**p = 0.0013) (n = 3 mice/group) and decreased with ML351 treatment. (C) Double labeling of NLRP1 (red) and neurons (NeuN; neuronal marker) (green) showed that NLRP1 is colocalized with the NeuN signal. Some of the overlapping markings are shown with arrows.