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. 2023 Sep 26;17:1277268. doi: 10.3389/fncel.2023.1277268

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Copyright © 2023 Cakir-Aktas, Bodur, Yemisci, van Leyen and Karatas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NLRP3/12/15-LOX, Caspase-1, and NLRP3/Caspase-1 immunolabelling at 6-h, 24-h, or 72-h following I/R. Nuclei were stained with Hoechst-33258 (blue) (40X, scale bar = 25 μm). (A) Representative images of NLRP3 (green), 12/15-LOX (red), and Hoechst-33258 (blue) were overlapped in the third panel (n = 3). Double labeling of NLRP3 and 12/15-LOX showed that there was a strong colocalization that acted in parallel and decreased in ML351-treated brains at all time points of I/R. (B) Caspase-1, a downstream effector of inflammasome activation, staining indicated that caspase-1 is increased mainly at 24-h and 72-h of I/R. (C) NLRP3 and caspase-1 double labeling was performed at 24-h of I/R brains, as prominent caspase-1 activation was observed at this time point, which revealed that caspase-1 positive cells were also positive for NLRP3.