Fig. 1.

Stage 1: After the vmPFC is dissected from a coronal slice containing the frontal lobe, 100 mg of vmPFC tissue is more precisely microdissected using a sterile blade or scalpel. Afterwards, the vmPFC tissue sample is dissociated in Homogenization Buffer using the GentleMACS™ Dissociator. Stage 2: Following dissociation, the sample is centrifuged at 3200 g for 30 mins in order to pellet dissociated microvessels. Additional downstream applications can be applied using microvessel-enriched pellets as shown in stages 3–6 by BCIP/NBT detection, immunofluorescent visualization, total RNA extraction, and total protein extraction, respectively. Image generated using BioRender.