Fig. 2.

a-g) Overview of experimental steps taken to collect and resuspend pelleted microvessels (BCIP/NBT staining is omitted). a) 100 mg of vmPFC tissue submerged in 2 ml of Homogenization Buffer. b) 100 mg of vmPFC tissue after homogenization. c) Lysate topped-up to 10 ml with Homogenization Buffer. d) Lysate transferred to 15 ml falcon tube. e-f) After centrifugation at 3200g for 30 min, a microvessel-enriched pellet forms at the bottom of the tube. g) microvessel-enriched pellet is resuspended in 500 ul of PBS. h-i) Output of experimental steps when BCIP/NBT staining is used. h) Example of BCIP/NBT-stained microvessel pellet, which lends a purple colour to the pellet. i) BCIP/NBT-stained microvessels trapped within the meshwork of a cellular strainer.