Figure 2.

Modeling of the GlcNAc₂Man₉ glycan bound to the CtUGGT_GT24 domain

(A) Man ”G″ placement next to the UDP-Glc binding site, in an orientation suitable for the nucleophilic attack of its O3 oxygen to the glucose anomeric center (red dashed line), to yield the β(1–3) Glc-Man bond.

(B) GlcNAc₂Man₉Glc₃ glycan nomenclature and final model of the GlcNAc₂Man₉Glc₁ glycan docked onto the CtUGGT_GT24 domain. Saccharide moieties are color-coded according to the scheme on the left hand side.⁴⁰

(C and D) The docked GlcNAc₂ moiety of the Man₉GlcNAc₂N-linked glycan and 8-OH-Q share a binding pocket.