Figure 3.

Heterozygous and homozygous mutations in Kmt2d promote tumor growth by P21

(A–C) Images of GFP stained (DAB) P12 sagittal sections from the hemispheres of SmoM2, SmoM2-Kmt2d^Cf/+, and SmoM2-Kmt2d^Cf/Cf animals. Scale bar: 1 mm. Green boxes indicate where the images in (A′–C′) were taken and black boxes where the images in (F–L′) are from. (A′–C″) Images of overlayed Hoechst (blue) and GFP (green) staining (A′–C′) and GFP (white) staining (A″–C″). Scale bar: 100 μm.

(D and E) Quantification of the percentage of the cerebellum sectional area taken up by the EGL in the hemispheres (D) and vermis (E) of P12 SmoM2, SmoM2-Kmt2d^Cf/+, and SmoM2-Kmt2d^Cf/Cf animals (n = 3 samples per genotype).

(F–H) Images of overlayed Hoechst (blue) and TUNEL (green) staining (or single channel TUNEL staining (F′– H′) in the EGL in the area indicated by the black box in (A–C). Scale bar: 250 μm.

(I) Quantification of the density of TUNEL+ cells in the EGL in the area of the hemispheres shown in (F–H) of SmoM2, SmoM2-Kmt2d^Cf/+, and SmoM2-Kmt2d^Cf/Cf mice (n = 3 samples per genotype).

(J′–L′) Representative images of overlayed Hoechst (blue) and EdU (red) staining or single channel EdU (red) staining in the EGL in the area indicated by the black box in (A–C). Scale bar: 250 μm.

(M) Quantification of the density of EdU+ cells in the EGL in the area of the hemispheres shown (J–L) of SmoM2, SmoM2-Kmt2d^Cf/+, and SmoM2-Kmt2d^Cf/Cf mice (n = 3 samples per genotype).

(N–P′) Images of GFP stained (DAB) P21 sagittal sections from the hemispheres (N–P) and vermis (N′–P′) of SmoM2, SmoM2-Kmt2d^Cf/+, and SmoM2-Kmt2d^Cf/Cf animals. Scale bar: 1 mm.

(Q and R) Quantification of the percentage of the cerebellum sectional area taken up by the EGL/lesion in the hemispheres (Q) and vermis (R) of P21 SmoM2, SmoM2-Kmt2d^Cf/+, and SmoM2-Kmt2d^Cf/Cf animals (n = 3 samples per genotype). All statistical significance was determined using an unpaired t-test comparing Kmt2d wildtype tumors to each of the Kmt2d mutants. Error bars: SD.