Figure 1.

Specific TAP-tagged translation and mRNA decay factorsbind to a validated set of interacting partners. Diagrammatic representation of the roles of the translation initiation factors eIF2 and eIF3, and mRNA decay factors Pat1 and Lsm1 in mRNA translation and turnover (A). TAP-affinity purifications using whole cell extracts from strains bearing eIF2γ-TAP, eIF3b-TAP, or eIF4E-TAP as a control. Western blots on total (T), unbound (UB) and TAP-immunoprecipitated (IP) samples using antibodies (α) to eIF2 and eIF3 subunits, as well as eIF4G1, Pab1p, and the ribosomal subunit Rps3p (B). As (B) except Lsm1-TAP, Pat1-TAP, and Prt1-TAP carrying strains were used and anti-myc antibodies were used to detect myc-tagged Dhh1p (C). eIF4G1, eukaryotic initiation factor 4F.