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. 2023 Oct 9;80(11):1209–1221. doi: 10.1001/jamaneurol.2023.3530

Figure 3. Measurement Oligomeric and Hyperphosphorylated Tau Species in Total Brain Homogenates and in Synaptosome Fractions.

Figure 3.

Synaptosomes derived from brains with dementia show a significant increase in tau oligomeric complex 1 (TOC1)–positive tau oligomers and of hyperphosphorylated AT270 phosphorylated (p)Thr181–positive tau compared with resilient and control brains. Western blot (WB) analyses of TOC1 plus tau oligomers in total brain tissue homogenates (A) and synaptosome extractions (B) were conducted by quantifying the signal intensity of the full lane labeled oligomers for each case in the native TOC1 WB. The WB analyses of synaptosome-enriched fractions from the visual cortex (B-D) total tau, as measured with the middle domain total tau antibody (Tau5), did not differ across dementia, resilient, and control brains (D). Analyses were performed for 31 total homogenates (6 controls, 10 resilient, 15 dementia) and 21 synaptosome extractions (4 controls, 5 resilient, 12 dementia). Black arrowheads indicate the bandwidth quantified for each WB analysis and respective antibodies. C indicates control brain (Braak stage 0-II); D, dementia brain (Braak stage III-IV); and R, resilient brain (Braak stage III-IV); RFU, relative fluorescence unit.