FIG. 3.

Effect of roxithromycin on neutrophil adhesion onto IFN-γ (100 ng/ml)-treated Bet-1A in vitro. Different concentrations of roxithromycin were added to Bet-1A epithelial cells simultaneously with IFN-γ (100 ng/ml) for 18 h. The purified neutrophils were then added with and without 30 min of pretreatment with FMLP (10⁻⁷ M). Roxithromycin showed a concentration-dependent inhibitory effect on neutrophil-epithelium adhesion at a concentration of no less than 1 μg/ml in FMLP-treated neutrophils (MPO assay) (a) and at a concentration of 25 μg/ml in naive neutrophils (b). For both panels, an asterisk represents a P of <0.05 compared to controls (ANOVA; n = 4). Error bars, standard deviations; OD, optical density.