REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse IgA antibody-HRP	Invitrogen	Cat# 62-6720
Anti-mouse IgG (Fab) antibody-HRP	Sigma	Cat# A9917; RRID: AB_258476
Anti-mouse-IgG (H+L) antibody-HRP	Invitrogen	Cat# 31430
Anti-Mouse IgG1 antibody-HRP	Invitrogen	Cat# PA1-74421
Anti-Mouse IgG2c antibody-HRP	Invitrogen	Cat# PA1-29288
Experimental models: Cell lines
hACE2/293T	Laboratory stock	N/A
HEK293F	Thermo Fisher Scientific	Cat# K900001
HEK293T	ATCC	Cat# CRL-3216; RRID: CVCL_0063
Chemicals, peptides, and recombinant proteins
Fat-free milk	Bio-Rad	Cat# 1706404
Polyetherimide (PEI)	Sigma	Cat# 919012
Recombinant DNA
pLenti-CMV-luciferase	Addgene	Cat# 17477
PS-PAX2	Addgene	Cat# 12260
Software and algorithms
Gen5	BioTek Instruments	N/A
GraphPad Prism 9	Graphpad Software	N/A
Bacterial and virus strains
Live SARS-CoV-2 Delta variant	GISAID	Accession No: EPI_ISL_2331496
Pseudotyped SARS-CoV-2 original strain	GenBank	Accession No: QHR63250.2
Pseudotyped SARS-CoV-2 BA1 subvariant	GISAID	Accession No: EPI_ISL_6795835
Pseudotyped SARS-CoV-2 BA2 subvariant	GISAID	Accession No: EPI_ISL_12030355
Pseudotyped SARS-CoV-2 BA5 subvariant	GISAID	Accession No: EPI_ISL_12043290
Pseudotyped SARS-CoV-2 XBB subvariant	GISAID	Accession No: EPI_ISL_15341139
Other
Aluminum adjuvant	InvivoGen	Cat# vac-alu-250
Fetal bovine serum (FBS)	R&D Systems	Cat# S11550
Luciferase Assay System	Promega	Cat# E1501
Monophosphoryl lipid A adjuvant	InvivoGen	Cat# vac-mplas
Ni-NTA Superflow	Qiagen	Cat# 1018401
nProtein A Sepharose 4 Fast Flow	GE Healthcare	Cat# 17-5280-02
Gibco™ Penicillin and Streptomycin	Thermo Fisher Scientific	Cat# 15140122
Poly(I:C) adjuvant	InvivoGen	Cat# tlrl-picw