Figure 6.

Characterization of the anti-Gram phage M13_Gram−

(A) Schematic representation of the M13 genetic engineering through co-transformation in E. coli of pPK_LPS phagemid and hyperphage genome.

(B–F) (B) Immunoblotting of the KNYSSSISSIRAC-pIII fusion (M13_Gram−) demonstrating incorporation in the purified virion. Visualization of purified M13_Gram− phage through (C) TEM (scale bar = 200 nm) and (D) AFM (scale bar = 600 nm). (E) M13_Gram− length distribution analyzed by AFM. (F) Selective tropism of engineered M13_Gram− to Gram-negative bacteria was proved through targeting assays performed on S. aureus (orange), P. aeruginosa (magenta), and A. baumannii (cyan). Data are represented as mean ± SD. ∗∗∗ = p < 0.001, ∗∗∗∗ = p < 0.0001.