REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-M13 g3p monoclonal antibody | New England BioLabs | Cat#E8033; RRID:AB_1559734 |
HRP-conjugated IgG goat anti-mouse IgG | Jackson | Cat#115-035-166; RRID:AB_2338511 |
Bacterial and virus strains | ||
E. coli Tg1 | DSMZ | 6056 |
E. coli DH5α | DSMZ | 6897 |
Acinetobacter baumannii ATCC 19606 | American Type Culture Collection | 19606 |
Pseudomonas aeruginosa PA14 | DSMZ | 19882 |
Staphylococcus aureus ATCC 29213 | American Type Culture Collection | 29213 |
M13KO7 Helper Phage | New England BioLabs | NO315S |
Hyperphage M13 KO7ΔpIII | PROGEN | PRHYPE |
Chemicals, peptides, and recombinant proteins | ||
Rose Bengal (RB) | Merck | 330000 |
EDC | Merck | 03450 |
Tetrazolium Violet | Merck | T0138 |
NHS | Merck | 130672 |
ABMDMA | Merck | 75068 |
Amplex Red | Merck | 90101 |
HRP | Merck | P6782 |
Critical commercial assays | ||
PowerUp™ SYBR™ Green Master Mix for qPCR | Thermo Fisher Scientific | A25742 |
LIVE/DEAD™ BacLight™ Bacterial Viability Kit | Thermo Fisher Scientific | L7007 |
Deposited data | ||
pPK_LPS | This study | GenBank: OQ803482 |
pPKC20 | This study | GenBank: OQ803483 |
M13KO7Δg3pΔoriF1 | This study | GenBank: OQ803484 |
Plasmids and phagemids | ||
pComb3XSS | Addgene | 63890 |
pPK_LPS | This study | GenBank: OQ803482 |
pPK_C20 | This study | GenBank: OQ803483 |
M13KO7Δg3pΔoriF1 | This study | GenBank: OQ803484 |
Oligonucleotides | ||
Primer: forward amplification C20 (AD251) GTTTTGAGCTCCAGGTGCAG |
This study | N/A |
Primer: reverse amplification C20 (AD252) GTTTTACTAGTACTAACGGTAACCTGG |
This study | N/A |
Primer: forward amplification Li005 (AD300) CAAAAACTATTCGAGCTCGATTAGCAGCATTCGTGCGTGCA |
This study | N/A |
Primer: reverse amplification Li005 (AD301) CTAGTGCACGCACGAATGCTGCTAA TCGAGCTCGAATAGTTTTTGAGCT |
This study | N/A |
Primer: forward M13 F1ori (AD046) AGGCTGCACCGGTGCGTCAG |
This study | N/A |
Primer: reverse M13 F1ori (AD047) ACACCCGCCGCGCTTAATGCG |
This study | N/A |
Primer: forward qPCR (AD211) TCCGGCCTTGCTAATGGTAA |
This study | N/A |
Primer: reverse qPCR (AD212) CGCAAAGACACCACGGAATA |
This study | N/A |
Software and algorithms | ||
BLAST | https://blast.ncbi.nlm.nih.gov/BlastAlign.cgi | v 2.6.0 |
MUSCLE | https://www.ebi.ac.uk/Tools/msa/muscle/ | v 3.8.31 |
ImageJ | GitHub | v 1.43t |
NanoScope Analysis | Bruker corporation | v 1.80 |
MATLAB | Mathworks | v 9.7 R2019b |
CytExpert | Beckman | v 2.4 |
FlowJo | BD Biosciences | v 10 |
Prism | GraphPad | v 10 |