Figure 2.

Amino acid changes along the primary sequence of BlaEC enzymes, shown with evolutionary conservation. The observed amino acid substitutions in 2280 BlaEC enzyme variants are above the BlaEC-1 enzyme sequence. Proline substitutions are highlighted in red. The colored bars characterize the general categories of amino acids. Residues without bars represent zero identified substitutions. Numbers are read vertically and indicate SANC-based amino acid residue positions. Above the observed amino acid substitutions are the change frequencies for each position. Alpha-helices (blue barrel), 3_10-helix (green barrel), and beta-strand or beta-bridge (yellow arrows).