Table 1.

Primer sequences used to detect the bla_EC genes by PCR.

Primer name	Sequence (5′–3′ direction)a	Target (variants)	Amplicon size (bp)	Tm (°C)	Reference
BlaEC-F1/R1	KAAATCCTCAAGCGACKTGC, AATGGTCGACTTYACACCA	206 specific BlaEC variants (BlaEC-1/-42/-74/-100/-101/-104/-122/-123/-144/-169/-182/-188/-211/-250/-266/-287/-289/-330/-331/-336/-337/-343/-372/-400/-401/-437/-443/-451/-452/-460/-492/-547/-548/-559/-659/-671/-706 to -709/-728/-742/-786/-791/-816/-859 to -861/-892/-899/-928/-979/-1041/-1047/-1056/-1059/-1077/-1079/-1081/-1100/-1101/-1110/-1115/-1116/-1126/-1144/-1145/-1201/-1202/-1211/-1339/-1367/-1387/-1410/-1433/-1440/-1489/-1497/-1520/-1523/-1546/-1601/-1631/-1672/-1673/-1683/-1785 to -1787/-1796/-1852/-1879/-1881/-1897/-1923 to -1925/-1927 to -1931/-1933/-1948/-1995/-1996/-2008/-2049/-2082/-2101 to -2103/-2105/-2106/-2108 to -2120/-2122/-2125/-2140/-2142 to -2155/-2159 to -2176/-2178 to -2187/-2189 to -2199/-P1 to -P4/-P6/-P9 to -P11/-P48 to -P50/-P69 to -P73/-P75 to -P81) and many others*	307	55	Mlynarcik et al. (2021a)
BlaEC-F2/R2	ACAAAATACTGGCCTGAACT, TTTTTGTTAGCCAGCATCAC	139 specific BlaEC variants (BlaEC-14/-17/-18/-40/-43/-44/-47/-49/-60/-71/-75/-107/-108/-116/-117/-132/-142/-151 to -153/-155/-163/-203/-241 to -243/-248/-252/-253/-275/-286/-298/-310/-326/-367/-377 to -379/-381 to -388/-402 to -415/-417 to -419/-421 to -423/-426/-427/-438/-446/-447/-467/-468/-471/-473 to -475/-484/-485/-488/-489/-519/-639/-658/-669/-754 to -767/-769 to -773/-776/-777/-792/-896/-989/-1102/-1104/-1130/-1205/-1227/-1292/-1293/-1370/-1446/-1449/-1485/-1593/-1595/-1596/-1615/-1676/-1684/-1818/-1820/-1830–1832/-1912/-1957/-1993/-2015/-2043/-2044/-2077/-2100/-2107) and many others*	761	52	This study
BlaEC-F3/R3	TACTTTACCTGGGGCTATGC, CTTCAGCATCTAACGCCC	BlaEC (9 variants: BlaEC-380/-416/-420/-768/-774/-775/-1908/-2177/-2188)	541	55	This study

^aFor degenerate primers: K = G or T; Y = C or T.

^*Primer pairs can detect an additional 1927 BlaEC variants.