Table V.

Immunoprecipitation of murine neutrophils using anti-NOS1AP Abs

	RAGE	NOS2	SrcK	ASC	TLR4	CD36	130CAS	Filamin
20 mM	2.6 ± 0.2	1.7 ± 0.2	2.8 ± 0.4	1.7 ± 0.3	2.1 ± 0.4	2.8 ± 0.5	2.2 ± 0.6	2.6 ± 0.4
Small inhibitory RAGE 20 mM	0.3 ± 0.2	0.9 ± 0.2	1.2 ± 0.2	0.9 ± 0.2	1.0 ± 0.2	1.2 ± 0.2	1.0 ± 0.2	1.2 ± 0.3
Small inhibitory TLR4 20 mM	0.7 ± 0.2	0.8 ± 0.3	1.1 ± 0.3	0.9 ± 0.3	0.3 ± 0.2	1.0 ± 0.4	0.8 ± 0.3	0.7 ± 0.1
CD36KO 20 mM	0.9 ± 0.2	0.8 ± 0.3	1.2 ± 0.3	1.1 ± 0.3	0.9 ± 0.4	0.1 ± 0.2	0.9 ± 0.3	0.9 ± 0.1

Data are the fold change in mean band densities ± SD (n = 4 for all groups) for cells incubated in 20 mM glucose for 2 h normalized to NOS1AP loading and to the value of the protein band density of cells incubated at 5.5 mM glucose. Where indicated, cells had first been incubated overnight with siRNA to deplete RAGE or TLR4 or cells from CD36 KO mice. Bold numbers are significantly different from control p < 0.05 ANOVA.