Figure EV1. Cycle threshold (CT) of miRNAs in the serum of sham and septic mice.

After 24 h of sham or CLP operation in mice, serum samples were collected and miRNAs were extracted. PCR array was performed in an Applied Biosystems StepOnePlus real‐time PCR machine under the thermal profile of 95°C for 10 min and followed by 33 cycles of 95°C for 15 s and 60°C for 1 min. Caenorhabditis elegans miRNA 39‐3p was spiked in and used to normalize comparative cycle threshold (CT) values.