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. 2019 Nov 14;21(1):e48075. doi: 10.15252/embr.201948075

Figure 4. MicroRNA 130b‐3p attenuates eCIRP‐induced pro‐inflammatory responses in macrophages in vitro .

Figure 4

  • A, B
    RAW264.7 cells (1 × 106 cells/ml) were treated with PBS or rmCIRP (1 μg/ml) with various doses (10, 100, and 1,000 nM) of miRNA 130b‐3p mimic. After 24 h of stimulation, supernatants were collected and assessed for TNF‐α and IL‐6 by ELISA.
  • C
    A total of 1 × 106 cells/ml of peritoneal macrophages were treated with PBS or rmCIRP (1 μg/ml) with various doses (10, 100, and 1,000 nM) of miRNA 130b‐3p mimic. After 24 h of stimulation, supernatants were collected and assessed for TNF‐α by ELISA. rmCIRP and miRNAs mimics were combined 30 min prior to stimulation to macrophage cells.
Data information: Data are expressed as means ± SE (n = 4–6 samples in each group) and compared by using one‐way ANOVA and SNK method (*P < 0.05 vs. PBS; # P < 0.05 vs. rmCIRP treatment).