-
A, B
RAW264.7 cells (1 × 106 cells/ml) were treated with PBS or rmCIRP (1 μg/ml) with various doses (10, 100, and 1,000 nM) of miRNA 130b‐3p mimic. After 24 h of stimulation, supernatants were collected and assessed for TNF‐α and IL‐6 by ELISA.
-
C
A total of 1 × 106 cells/ml of peritoneal macrophages were treated with PBS or rmCIRP (1 μg/ml) with various doses (10, 100, and 1,000 nM) of miRNA 130b‐3p mimic. After 24 h of stimulation, supernatants were collected and assessed for TNF‐α by ELISA. rmCIRP and miRNAs mimics were combined 30 min prior to stimulation to macrophage cells.
Data information: Data are expressed as means ± SE (
n = 4–6 samples in each group) and compared by using one‐way ANOVA and SNK method (*
P <
0.05 vs. PBS;
#
P <
0.05 vs. rmCIRP treatment).