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. 2019 Nov 14;21(1):e48075. doi: 10.15252/embr.201948075

Figure 6. MicroRNA 130b‐3p mimic protects mice from sepsis.

Figure 6

  • A–I
    Sepsis was induced in mice by CLP. Mice were injected with either miRNA 130b‐3p mimic at the concentration of 125 nM (12.5 μl of 1,000 nM miRNA 130b‐3p mimic) in 100 μl of PBS or equal volume of PBS as vehicle at 2, 5, and 10 h after CLP via intraperitoneal injection. After 20 h of CLP, blood samples and lung tissue were harvested to assess tissue injury markers (B) ALT, (C) LDH, inflammatory cytokines (D) TNF‐α and (E) IL‐6 in serum, and the expression of (F) TNF‐α, (G) IL‐6, (H) MIP‐2, and (I) KC in the lungs. ALT, alanine aminotransferase; CLP, cecal ligation and puncture; IL‐6, interleukin‐6; KC, keratinocyte chemoattractant; LDH, lactate dehydrogenase; MIP‐2, macrophage inflammatory protein‐2; TNF‐α, tumor necrosis factor‐α.
  • J
    Representative images of H&E‐stained lung tissue at 200×. Scale bar, 100 μm.
  • K
    Lung injury score calculated at 200×. n = 5 high‐powered fields/group. Each group contains five mice. CLP, cecal ligation and puncture.
Data information: Data are expressed as means ± SE (A‐I: n = 10 mice/group) and compared by one‐way ANOVA and SNK method (A‐I: *P < 0.05 vs. PBS‐treated mice; # P < 0.05 vs. vehicle‐treated septic mice; K: *P < 0.05 vs. sham and # P < 0.05 vs. vehicle mice).