A–ISepsis was induced in mice by CLP. Mice were injected with either miRNA 130b‐3p mimic at the concentration of 125 nM (12.5 μl of 1,000 nM miRNA 130b‐3p mimic) in 100 μl of PBS or equal volume of PBS as vehicle at 2, 5, and 10 h after CLP via intraperitoneal injection. After 20 h of CLP, blood samples and lung tissue were harvested to assess tissue injury markers (B) ALT, (C) LDH, inflammatory cytokines (D) TNF‐α and (E) IL‐6 in serum, and the expression of (F) TNF‐α, (G) IL‐6, (H) MIP‐2, and (I) KC in the lungs. ALT, alanine aminotransferase; CLP, cecal ligation and puncture; IL‐6, interleukin‐6; KC, keratinocyte chemoattractant; LDH, lactate dehydrogenase; MIP‐2, macrophage inflammatory protein‐2; TNF‐α, tumor necrosis factor‐α.