FIGURE 2.

Puerarin alleviates APAP‐induced oxidative stress in HepG2 cells. HepG2 cells were pretreated with 15, 30 and 60 μmol L⁻¹ Pue for 1 h, and then incubated with 10 mmol L⁻¹ APAP for 24 h. DCFH‐DA fluorescent probe was added into the culture medium. Representative images were taken under a fluorescence microscope at 200× (a), and fluorescence intensity was detected with a fluorescence microplate reader (b). The cells were lysed by ultrasonic wave and the contents of MDA (c), CAT (d), T‐SOD (e) and GSH (f) in cell lysate were detected by microplate colorimetry. The data represent the mean ± SD of at least three independent experiments. *p < .05, **p < .01 versus Blank control group; ^# p < .05, ^## p < .01 versus APAP group.