FIGURE 4.

Protective effect of puerarin on acute liver injury induced by APAP in mice. After 7 days of feeding, mice were given different doses of Pue (200, 400 and 800 mg kg⁻¹) for 10 days, and then, 300 mg kg⁻¹ APAP was intraperitoneally injected to establish the model of acute liver injury. (a) The liver index was measured. (b) The contents of ALT, AST, TBIL and γ‐GT in serum of mice were measured. (c) The representative HE staining liver samples were observed under a 100× and 400× microscope. The liver homogenate was prepared, and the supernatant was taken after centrifugation. The contents of MDA (d), CAT (e), T‐SOD (f) and GSH (g) in supernatant were detected by microplate colorimetry. The data represent the mean ± SD, n = 10. *p < .05, **p < .01 versus. Blank control group; ^# p < .05, ^## p < .01 versus APAP group.