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. 2023 Oct 6;18:5651–5670. doi: 10.2147/IJN.S426849

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© 2023 Li et al.

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Characterization of MUC1-C shRNA@Fe₃O₄ MNPs. Appearance (A) and TEM image (D) of the prepared MUC1-C shRNA@Fe₃O₄ MNPs. DSL Size distribution of Fe₃O₄ MNPs (B and C) and MUC1-C shRNA@Fe₃O₄ MNPs (E and F). Tyndall effect of MUC1-C shRNA@Fe₃O₄ MNPs (G). Zeta potential of MUC1-C shRNA@Fe₃O₄ MNPs (H). Encapsulation efficiency at different shRNA/MNPs ratios (I). TEM elemental mapping analysis of MUC1-C shRNA@Fe₃O₄ MNPs (J). Raman spectrum of MUC1-C shRNA, Fe₃O₄ MNPs, and MUC1-C shRNA@Fe₃O₄ MNPs (K). Release profiles of Fe²⁺ from MUC1-C shRNA@Fe₃O₄ MNPs at different pH values with or without AMF (L). The heating curves of MUC1-C shRNA@Fe₃O₄ MNPs with different concentrations (50–250μg/mL) (M). Hemolysis test at different concentrations (MUC1-C shRNA@Fe₃O₄ MNPs) (n=3) (N and O). Hemolysis test (200μg/mL, n=3) (P and Q). Error bars represent means ± SD. TEM scale bar: 50nm.