Fig 4. Volcano plots showing comparisons of serum profiles between treatment responsive and treatment non-responsive subjects for each clinical outcome group.

(A) MMS status (responsive Yes vs non-responsive No) (B) change in (Δ) QMG (responsive > 40% vs. non-responsive ≤ 0%), (C) change in (Δ) MG-ADL (responsive > 40% vs. non-responsive ≤ 0%), and (D) Responders vs Non-Responders. Gray dots represent metabolites and lipids that were not significantly altered between treatment responsive and non-responsive subjects, teal dots represent metabolites with a fold change greater than 2-fold (cutoff), purple dots represent metabolites and lipids with a significant p value (p < 0.05) and yellow dots indicates those metabolites and lipids with both a significant fold change and p-value. Differences between outcome groups were tested using the non-parametric Mann Whitney U test and FDR correcting for multiple testing with Benjamini-Hochberg procedure two-sided. P-values < 0.05 were regarded as statistically significant. Individual p-values for each metabolite and lipid are found in S2 Table.