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Lewis lung carcinoma (LL-2-Luc-M38) cells were injected via the tail vein into immunocompetent C57BL/6 and immunodeficient NSG mice. Within each population, one control group was kept without anesthesia administration (control). One group was administered volatile anesthesia with isoflurane, and one group was administered intraperitoneal ketamine. Using bioluminescence methods, the mice were imaged on days 0, 3, 10, and 14 post tumor cells injection.
