TABLE 2.
Synthetic oligonucleotide primers
| Primer | Nucleotide sequence | Coordinatesa |
|---|---|---|
| 94 | 5′-GAAAATGTCAAGGACTT-3′ | 72–88 |
| 211 | 5′-CGTTCCTTGTCCTGCT-3′ | 5542–5527 |
| 212 | 5′-TCGGGGACTATTACTA-3′ | 2173–2188 |
| 220 | 5′-CATCAATCACAATCTC-3′ | 5025–5010 |
| 221 | 5′-TGGTGCGGTAGAGTGG-3′ | 4131–4116 |
| 274 | 5′-AACCTGTGGTTATGTAT-3′ | 3407–3391 |
| 300 | 5′-GGGCTATACTTTAATAG-3′ | 1–17 |
| 1727 | 5′-GGGGTCGAGTTTGTCAAG-3′ | 6338–6321 |
| 3277b | 5′-AGTATTCCGCAAAGGTTTTTCTTCTGCGG-3′ | 2220–2200 |
| 4675 | 5′-AATCAAGCAGGACAAGGAACGA-3′ | 5522–5543 |
| 4676 | 5′-TGAAGTCCTTGACATTTTCTTA-3′ | 86–75 |
a
All primers were derived from Tn4451, the nucleotide sequence of which was published previously (5).
b
Note that the first 8 nucleotide bases at the 3′ end of this oligonucleotide primer are not derived from Tn4451.