TABLE 4.
Complementation of Tn4451tnpXΔ1 in E. coli
| Straina | pBS or pUC Replicon:
|
% Stabilityb | |
|---|---|---|---|
| Plasmid | Genotypec | ||
| JIR5509 | pUC18 | 100.0 ± 0 | |
| JIR2771 | pJIR639 | tnpX+ (Tn4451) | 48.0 ± 4.5 |
| JIR5510 | pJIR1488 | tnpX+ (Tn4453a) | 4.4 ± 1.3 |
| JIR5511 | pJIR1489 | tnpX+ (Tn4453b) | 14.0 ± 5.1 |
a
In addition to the pBluescript-II KS (pBS), pUC18, or pUC19 (pUC) vector indicated, each strain carried the pSU39-based recombinant plasmid pJIR773, which carried Tn4451tnpXΔ1 (5).
b
Each value is an average of three independent experiments and refers to the percentage of kanamycin-resistant colonies able to grow in the presence of chloramphenicol.
c
Refers to the tnpX gene carried by the recombinant pBS- or pUC-derived plasmid only.