Fig. 2.

Somatic embryogenesis of Eleutherococcus sessiliflorus: A Embryogenic callus developed from leaf explants on MS medium with 1 mg L⁻¹ 2,4-D and 30 g L⁻¹ sucrose after 6 weeks of culture; B globular embryos developed from an embryogenic callus on MS medium with 1 mg L⁻¹ 2,4-D and 30 g L⁻¹ sucrose after 8 weeks of culture; C heart-staged and D torpedo-staged embryos developed from an embryogenic callus upon transfer to an MS medium with 30 g L⁻¹ sucrose after 10 and 12 weeks of culture, respectively; E germinated embryos upon transfer to an MS medium supplemented with 30 g L⁻¹ sucrose and 4 mg L⁻¹ GA₃; and F plantlets growing in the greenhouse 2 months after transfer to garden soil (Cocopeat 51%, Peat moss 10%, Vermiculite 13%, Humic acid 0.1%, Perlite 15%, Zeolite 10%, Fertilizer 0.4%; Shinsung Mineral Co., Ltd., Dunchon-aero, Republic of Korea)