Table 2.
Impact of glutathione on total soluble proteins, fresh weight of roots, fresh weight of shoots, and dry mass of broccoli seedlings under Pb stress
| Treatments | Shoot fresh weight (g) |
Root fresh weight (g) |
Total fresh weight (g) |
Shoot dry weight (g) |
Root dry weight (g) |
Total dry Weight (g) |
Total soluble proteins (mg g−1 FW) |
|---|---|---|---|---|---|---|---|
| C | 5.6 ± 0.35bc | 1.95 ± 0.12ab | 7.55 ± 0.45b | 1.77 ± 0.08a | 0.27 ± 0.10b | 2.05 ± 0.15ab | 14.5 ± 0.12b |
| Pb | 3.75 ± 0.28a | 1.52 ± 0.04a | 5.25 ± 0.25a | 0.57 ± 0.27a | 0.12 ± 0.02a | 1.7 ± 0.29a | 9.42 ± 0.41a |
| GSH1 | 5.75 ± 0.45bc | 1.75 ± 0.23a | 7.51 ± 0.44b | 1.97 ± 0.34ab | 0.25 ± 0.02ab | 2.22 ± 0.33abc | 17.65 ± 0.34c |
| GSH2 | 6.52 ± 0.34c | 2.75 ± 0.27 cd | 9.27 ± 0.26c | 3.12 ± 0.11c | 0.32 ± 0.02b | 3.45 ± 0.11d | 22.4 ± 0.42e |
| GSH3 | 6.3 ± 0.17c | 2.4 ± 0.36bc | 8.7 ± 0.46bc | 2.55 ± 0.15bc | 0.25 ± 0.02ab | 2.8 ± 0.13bc | 20.43 ± 0.51d |
| GSH1 + Pb | 4.87 ± 0.42b | 2.67 ± 0.13 cd | 7.55 ± 0.52b | 2.57 ± 0.31bc | 0.23 ± 0.02ab | 2.8 ± 0.29bcd | 16.32 ± 0.41bc |
| GSH2 + Pb | 6.02 ± 0.37c | 3.27 ± 0.12d | 9.30 ± 0.47c | 2.75 ± 0.30bc | 0.29 ± 0.03b | 3.04 ± 0.32d | 18.44 ± 0.43cd |
| GSH3 + Pb | 5.97 ± 0.10c | 3.15 ± 0.08d | 9.12 ± 0.12c | 2.62 ± 0.25bc | 0.28 ± 0.02b | 2.91 ± 0.25cd | 17.54 ± 0.25c |
Data display means ± SE of 4 replicates. Non-identical letters indicated significant differences between the treatments at P ≤ 0.05. C = un-contaminated control, Pb = contaminated control (300 mg/kg Pb), GSH1 = 25 μM Glutathione, GSH2 = 50 μM Glutathione, GSH3 = 75 μM Glutathione, Pb + GSH1 = 300 mg/kg Pb + 25 μM GSH, Pb + GSH2 = 300 mg/kg Pb + 50 μM GSH, Pb + GSH3 = 300 mg/kg Pb + 75 μM GSH