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. 2023 Oct 10;14(10):664. doi: 10.1038/s41419-023-06202-3

Fig. 5. Depletion of Drp1 increases glycogen storage in vivo.

Fig. 5

A Sh-Ctrl and sh-Drp1 PT130 cells were injected subcutaneously into NSG mice. Tumor tissues from both groups were analyzed for the expression of Drp1, GYS1 and β-actin using western blot. B The relative GYS1 levels in sh-Ctrl and sh-Drp1 tumors detected by western blot as shown in (A) were quantified by normalizing GYS1 to β-actin. Data were presented as mean ± SD (n = 4, *p < 0.05). C Representative images were obtained from sh-Ctrl and sh-Drp1 tumor sections stained with the anti-glycogen antibody. Scale bar, 200 μm. D The percentage of glycogen positive cells were quantified in tumors from four mice of each group using the HALO software. Data were presented as mean ± SD (n = 4, **p < 0.01). E TCGA COAD RNA-seq dataset was first used to identify genes that have positive or negative correlations with Drp1 (gene name: DNM1L) expression. The GSEA was then performed to determine if DNM1L expression is associated with gene sets in the REACTOME collection. The name of the gene sets and the corresponding normalized enrichment score (NES) and false discovery rate (FDR) are listed in the graph (the cutoff for significance is set for FDR < 0.05).