Table 1.
List of uncommon mutations in the spike gene detected in wastewater samples in >1.0% prevalence, and <1.0 prevalence in sequences from clinical cases worldwide from GISAID
| Mutation | Number of samples detected in Wastewater (N = 325) |
Prevalence in clinical cases in South Africa (N = 24 384) |
Prevalence in clinical cases Worldwide (N = 7 744 348) |
Wastewater sites |
|---|---|---|---|---|
| S50L | 9 (2,7692%) | 4 (0,0164%) | 855 (0,0110%) | Central eThekwini (3), Northern ethekwini (2), EastBank (2), Rooiwal (1), Hartbeesfontein (1) |
| H66Y | 5 (1,5384%) | 307 (1,2590%) | 2 439 (0,0315%) | Brickfield (2), Kwanobuhle (2), Rooiwal (1) |
| T250S | 34 (10,4615%) | 1 359 (5,5733%) | 1 706 (0,0220%) | Daspoort (8), Rooiwal (6), Hartbeesfontein (4), Central eThekwini (3), Northern Johannesburg (2), Northern eThekwini (2), Zandvliet (2), Bloemspruit (2), Sterkwater (2), Vlakplaats (1) |
| A288T | 9 (2,7692%) | 0 | 26 (0,0003%) | Northern eThekwini (2), Daspoort (2), Rooiwal (1), Mdantsane (1), Zandvliet (1) |
| K444T | 4 (1,2308%) | 0 | 125 (0,0016%) | Central eThekwini (3), Northern eThekwini (1) |
| Q498H | 4 (1,2308%) | 0 | 74 (0,0010%) | Central eThekiwnin (2), Zandvliet (1), Eastbank (1) |
| D627H | 27 (8,3077%) | 1 (0,0041%) | 372 (0,0048%) | Goudkoppies (4), Bloemspruit (4),Daspoort (4), Mdantsane (3), Sterkwater (3), Vlakplaats (2), Northern eThekwini (1), Northern Johannesburg (1), Rooiwal (1), Borcheds Quarry (1), Kwanobuhle (1), Central eThekwini (1) |
| L828F | 11 (3,3846%) | 3 (0,0123%) | 171 (0,0022%) | Central eThekwini (3), Zandvliet (3), Northern eThekwini (1), Northern Johannesburg (1), Hartbeesfontein (1), Rooiwal (1), Bloemspruit (1) |
| T859N | 13 (4,0000%) | 384 (1,5748%) | 30 185 (0,3898%) | Hartbeesfontein (4), Daspoort (2), Zandvliet (1), Vlakplaats (1), Central eThekwini (1), Rooiwal (1), Goudkoppies (1), Northern eThekwini (1), Sterkwater (1) |
| Q1201K | 4 (1,2308%) | 12 (0,0492%) | 2 876 (0,0371%) | Central eThekwini (3), Zandvliet (1) |