Table 1.
Details of antibody used in experiments.
| Antigen | Catalog number | Dilution | Source | Species |
|---|---|---|---|---|
| IF | ||||
| IL-33 | 66235-1-Ig | 1:200 | Proteintech | Mouse |
| Vimentin | 10366-1-AP | 1:50 | Proteintech | Rabbit |
| Cytokeratin 7 | 15539-1-AP | 1:50 | Proteintech | Rabbit |
| CD11b | 66519-1-Ig | 1:200 | Proteintech | Mouse |
| CD68 | 28058-1-AP | 1:200 | Proteintech | Rabbit |
| WB | ||||
| β-actin | 60008-1-Ig | 1:20,000 | Proteintech | Mouse |
| IL-33 | 66235-1-Ig | 1:1000 | Proteintech | Mouse |
| ST2 | 60112-1-Ig | 1:20,000 | Proteintech | Mouse |
| ACSL4 | 22401-1-AP | 1:1000 | Proteintech | Rabbit |
| SLC7A11 | 26864-1-AP | 1:1000 | Proteintech | Rabbit |
| GPX4 | 14432-1-AP | 1:5000 | Proteintech | Rabbit |
| ATF3 | DF3110 | 1:1000 | Affinity | Rabbit |
| JNK | 66210-1-Ig | 1:5000 | Proteintech | Mouse |
| p-JNK | 80024-1-RR | 1:1000 | Proteintech | Rabbit |
| p38 | 14064-1-AP | 1:1000 | Proteintech | Rabbit |
| p-p38 | 28796-1-AP | 1:1000 | Proteintech | Rabbit |
| GAPDH | 10494-1-AP | 1:5000 | Proteintech | Rabbit |
| IHC | ||||
| IL-33 | 12372-1-AP | 1:200 | Proteintech | Rabbit |
| ST2 | 60112-1-Ig | 1:200 | Proteintech | Mouse |
| ATF3 | DF3110 | 1:100 | Affinity | Rabbit |
| ST2 (Mouse) | 11920-1-AP | 1:400 | Proteintech | Rabbit |
| SLC7A11 (Mouse) | 26864-1-AP | 1:200 | Proteintech | Rabbit |
WB western blot, IHC immunohistochemistry, IF Immunofluorescence, IL-33 interleukin-33, IL1R-L1 ST2 interleukin receptor-like 1, ACSL4 acyl-CoA synthetase long-chain family member 4, SLC7A11 solute carrier family 7 member 11, GPX4 glutathione peroxidase 4, ATF3 activating transcription factor, JNK c-Jun N-terminal protein kinase, p-JNK phosphorylated c-Jun N-terminal protein kinase, p38/p38 MAPK p38 mitogen activated protein kinases, p-p38 phosphorylated p38 mitogen activated protein kinases.