Table 2.
Essential amino acids, macro- and trace elements, and selected fatty acids content (g kg−1 dry matter, DM) of the reference and experimental diets with inclusion of microalgae in substitution of the reference diet.
| Diet | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Reference | Chlorella vulgaris | Nannochloropsis oceanica | Tetradesmus obliquus | |||||||
| 0.5% | 1.0% | 1.5% | 0.5% | 1.0% | 1.5% | 0.5% | 1.0% | 1.5% | ||
| Essential amino acids | ||||||||||
| Arginine | 20.5 | 20.6 | 20.8 | 20.9 | 20.5 | 20.5 | 20.5 | 20.5 | 20.6 | 20.6 |
| Histidine | 6.43 | 6.45 | 6.46 | 6.48 | 6.42 | 6.42 | 6.41 | 6.42 | 6.41 | 6.40 |
| Lysine | 16.0 | 16.2 | 16.4 | 16.6 | 16.0 | 16.1 | 16.1 | 16.1 | 16.1 | 16.2 |
| Threonine | 10.5 | 10.6 | 10.7 | 10.8 | 10.5 | 10.5 | 10.6 | 10. 6 | 10.6 | 10.7 |
| Isoleucine | 9.63 | 9.69 | 9.75 | 9.81 | 9.64 | 9.65 | 9.66 | 9.66 | 9.69 | 9.72 |
| Leucine | 21.2 | 21.3 | 21.4 | 21.5 | 21.2 | 21.2 | 21.2 | 21.2 | 21.3 | 21.3 |
| Valine | 16.2 | 16.3 | 16.3 | 16.4 | 16.2 | 16.2 | 16.2 | 16.2 | 16.2 | 16.3 |
| Methionine | 3.68 | 3.72 | 3.75 | 3.79 | 3.69 | 3.70 | 3.73 | 3.70 | 3.72 | 3.74 |
| Methionine + cystine | 8.24 | 8.26 | 8.29 | 8.31 | 8.23 | 8.22 | 8.21 | 8.24 | 8.25 | 8.25 |
| Phenylalanine | 12.2 | 12.3 | 12.4 | 12.5 | 12.2 | 12.2 | 12.2 | 12.3 | 12.3 | 12.3 |
| Phenylalanine + tyrosine | 19.0 | 19.2 | 19. 4 | 19.6 | 19.0 | 19.1 | 19.1 | 19.1 | 19.2 | 19.3 |
| Fatty acids | ||||||||||
| C16:0 | 20.0 | 20.0 | 20.0 | 19.9 | 20.0 | 20.0 | 20.0 | 20.0 | 20.0 | 19.9 |
| C16:1 n-7 | 2.42 | 2.42 | 2.41 | 2.40 | 2.52 | 2.61 | 2.71 | 2.42 | 2.41 | 2.40 |
| C18:0 | 8.16 | 8.13 | 8.10 | 8.07 | 8.12 | 8.09 | 8.05 | 8.12 | 8.09 | 8.05 |
| C18:1 n-9 | 29.4 | 29.3 | 29.1 | 29.0 | 29.3 | 29.2 | 29.0 | 29.3 | 29.2 | 29.0 |
| C18:2 n-6 | 22.0 | 22.0 | 21.9 | 21.8 | 21.9 | 21.8 | 21.7 | 21.9 | 21.8 | 21.7 |
| C18:3 n-3 | 1.20 | 1.29 | 1.37 | 1.46 | 1.19 | 1.19 | 1.19 | 1.29 | 1.37 | 1.46 |
| C20:4 n-6 | 0.024 | 0.024 | 0.024 | 0.024 | 0.024 | 0.024 | 0.024 | 0.023 | 0.023 | 0.023 |
| C20:5 n-3 (EPA) | 0.028 | 0.028 | 0.029 | 0.029 | 0.117 | 0.206 | 0.295 | 0.028 | 0.029 | 0.030 |
| C22:6 n-3 (DHA) | 0.122 | 0.123 | 0.123 | 0.123 | 0.122 | 0.122 | 0.121 | 0.123 | 0.123 | 0.124 |
| Macro elements | ||||||||||
| Na | 3.13 | 3.12 | 3.10 | 3.09 | 3.30 | 3.48 | 3.65 | 3.12 | 3.12 | 3.11 |
| K | 6.03 | 6.04 | 6.05 | 6.07 | 6.09 | 6.16 | 6.22 | 6.06 | 6.10 | 6.13 |
| Mg | 0.430 | 0.435 | 0.435 | 0.436 | 0.451 | 0.469 | 0.486 | 0.435 | 0.435 | 0.436 |
| Ca | 15.5 | 15.4 | 15.3 | 15.3 | 15.4 | 15.3 | 15.2 | 15.4 | 15.3 | 15.3 |
| P | 21.0 | 21.0 | 21.0 | 21.1 | 21.0 | 20.9 | 20.9 | 21.0 | 21.0 | 21.0 |
| Ca:P ratio | 0.736 | 0.733 | 0.730 | 0.727 | 0.732 | 0.729 | 0.726 | 0.733 | 0.731 | 0.728 |
| Trace elements (mg kg−1 DM) | ||||||||||
| Fe | 135 | 138 | 140 | 143 | 136 | 137 | 137 | 149 | 164 | 178 |
| Mn | 45.6 | 46.2 | 46.8 | 47.4 | 45.6 | 45.5 | 45.5 | 45.9 | 46.2 | 46.6 |
| Cu | 13.5 | 13.6 | 13.6 | 13.7 | 13.5 | 13.5 | 13.5 | 13.5 | 13.4 | 13.4 |
| Zn | 177 | 178 | 179 | 180 | 176 | 176 | 175 | 177 | 176 | 176 |
| Se | 0.350 | 0.349 | 0.348 | 0.347 | 0.355 | 0.360 | 0.365 | 0.350 | 0.350 | 0.349 |