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. 2023 Aug 4;43(10):1097–1116. doi: 10.1002/cac2.12476

FIGURE 4.

FIGURE 4

MFSD2A inhibits TGFβ1 release from GC cells. (A) Expressions of TGFβ1, PVR, PD‐L1, and IL10 genes were analyzed in MFSD2A‐silenced or control MGC803 cells by transcriptome sequencing. (B) TGFβ1 mRNA expression in MFSD2A‐silenced or control MGC803 cells was examined by qRT‐PCR. (C‐E) TGFβ1 protein levels in the supernatant of MFSD2A‐silenced or control MGC803 cells (C), MFSD2A‐overexpressed MGC803 cells (D), or MFSD2A‐overexpressed MFC cells (E) was examined by ELISA. (F‐G) The expressions of MFSD2A and TGFβ1 in tumor tissues derived from MFSD2A‐overexpressed MFC cells‐bearing mice or mock control mice were analyzed by IHC. All values are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001, NS, not significant. Abbreviations: GC, gastric cancer; MFSD2A, Major Facilitator Superfamily Domain Containing 2A; TGFβ1, transforming growth factor‐beta 1; qRT‐PCR, quantitative real‐time polymerase chain reaction; ELISA, enzyme‐linked immunosorbent assay; PVR, PVR cell adhesion molecule; PCD‐L1, Programmed cell death ligand 1; IL10, interleukin 10; IHC, immunohistochemistry; SEM, standard error of the mean.