Figure 1.

GPI-ecto-TβRII is highly expressed on transduced Jurkat cells and ATCs

(A) Schematic representation of HA-DN and HA-GPI-ecto-TβRII construct design. The expression of any of the 4 tested GPI-ecto-TβRII constructs is several folds higher in Jurkat cells compared with HA-DN containing a natural transmembrane domain as seen in (B) flow cytometry or (C) displayed as mean fluorescence intensity (MFI) in bar graphs. (D) Flow cytometry dot plots or (E) MFI bar graphs of ATCs transduced with either HA-DN or one of four GPI-ecto-TβRII constructs. Bar graphs display the mean and SEM of at least 3 biological replicates. (F) Confocal microscopy of Jurkat cells transduced with either HA-DN or one of the four HA-GPI-ecto-TβRII constructs demonstrates transmembrane TβRII expression (red) in transduced cells.