Skip to main content
. 2023 Sep 27;14:1283161. doi: 10.3389/fimmu.2023.1283161

Figure 3.

Figure 3

Targeting of NTHi surface adhesins PE and Hap by collagen VI VWA domains. (A) Titration of bacterial solutions with radiolabeled collagen VI microfibrils. Serial dilutions of bacteria were used: 1% (2 × 109 cfu/ml), 0.5% (1 × 109 cfu/ml), 0.1% (2 × 108 cfu/ml), 0.01% (2 × 107 cfu/ml), and 0.001% (2 × 106 cfu/ml). Wild type bacteria are compared to isogenic mutants as indicated. (B–F) negative staining and transmission electron microscopy of collagen VI networks bound to the bacterial surface. Wild type (B) and Δhia (C) bacteria interact with collagen VI (arrows) as opposed to Δhpe (D), Δhap (E), and ΔhpeΔhap (F). PE (G) and Hap (H) are frequently colocalized with collagen VI on the bacterial surface as visualized by antibodies conjugated with 5 nm (PE and Hap, arrowheads) and 10 nm (collagen VI, arrows) colloidal gold, respectively. The scale bars represent 200 nm (B–F) and 100 nm (G, H).