Figure EV2. Effects of AAV9.NDP treatment on the retinal vessel morphology and barrier proteins.

• A–H
  Retinal cryosections at 2 months stained with IB4 (A–D, E–G, E–G′), anti‐PLVAP and anti‐CLDN5 antibodies (E″–G″) and anti‐EGFP antibody (E–H). Note the presence of three layers of vessels on WT (A‐A″, E‐E′) and P2‐L (C‐C″, G‐G′) groups and only one layer in Ndp‐KO (B‐B″, F‐F′) and P21‐H (D‐D″, H‐H′) groups. CLDN5 expression is visible in vessels in WT (E″′), P2‐L (G″′) and P21‐H (H″′) groups. Scale bar 50 μm (E–H).

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