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. 2023 Aug 17;15(10):e17094. doi: 10.15252/emmm.202217094

Figure 4. In silico identification of candidate FBXW7 targets reveals enrichment of Wnt pathway components including LEF1 and TCF7L2.

Figure 4

  1. Schematic indicating workflow for in silico identification of candidate FBXW7 targets carrying putative conserved phosphodegrons (CPDs). Experimentally validated FBXW7 CPDs (top, provided as Dataset EV8) were used to define a consensus motif (middle) for search against the human proteome to identify candidate FBXW7 targets (bottom).
  2. Dot plot showing enrichment analysis of 1,064 putative FBXW7 substrates against GO biological processes.
  3. Venn diagram showing overlap between putative FBXW7 substrates identified by in silico analysis and Wnt pathway components curated by the Gene Ontology Consortium (GO:0016055).
  4. Simplified representation of Wnt signalling pathway. Following stimulation by Wnt ligand, the GSK3β/Axin/APC destruction complex is inhibited and β‐catenin can translocate to the nucleus where it complexes with TCF/LEF family transcription factors to regulate Wnt target gene expression.
  5. Schematic of putative FBXW7 substrate LEF1 indicating position and conservation of candidate CPD, and phosphorylation sites corresponding to the ‘0’ and ‘+4’ positions curated in PhosphoSitePlus database.
  6. Corresponding schematic for putative FBXW7 substrate TCF7L2.

Source data are available online for this figure.