Figure 5. Flii regulates hematopoiesis through the Nlrp1 inflammasome in zebrafish.

• A–H
  Number of erythrocytes (A), neutrophils (B, F‐H), macrophages (C), and HSPCs (D), and caspase‐1 activity (E) in nlrp1 crispant larvae of 2 dpf obtained by injecting one‐cell stage embryos with standard, nlrp1 and/or flii crRNAs/Cas9 complexes. (G, H) Larvae were also treated by bath immersion with 100 μM of the caspase‐1 inhibitor VX‐765. Representative images of neutrophils in flii crispant larvae using Tg(mpx:eGFP) reporter line are shown in (G). Each dot represents one individual and the mean ± SEM for each group is also shown. P values were calculated using Student's t‐test. n.s., non‐significant; *P < 0.05; **P < 0.01; ****P < 0.0001.

Source data are available online for this figure.