Table 2. List of the Formulations Used for the Freeze-Drying of EVsa.
| excipient | concentration (w/v) (%) | abbreviation |
|---|---|---|
| physiologic solution | PS | |
| lactose | 5 | L5% |
| 10 | L10% | |
| sucrose | 5 | S5% |
| 10 | S10% | |
| trehalose | 5 | T5% |
| 10 | T10% | |
| H-β-cyclodextrin | 5 | C5% |
| 10 | C10% | |
| dextran | 5 | D5% |
| 10 | D10% | |
| lactose–dextran | 7–3 | L-D |
| sucrose–dextran | 7–3 | S-D |
| trehalose–dextran | 7–3 | T-D |
| dextran–PEG | 7–3 | D-PEG |
| lactose–PEG | 7–3 | L-PEG |
| sucrose–PEG | 7–3 | S-PEG |
| trehalose–PEG | 7–3 | T-PEG |
| mannitol | 5 | M5% |
| 10 | M10% | |
| glycine | 5 | G5% |
| 10 | G10% | |
| lactose–glycine | 7–3 | L-G |
| sucrose–glycine | 7–3 | S-G |
| trehalose–glycine | 7–3 | T-G |
| cells and EVs-free human serum | 10 | HS10% |
| 50 | HS50% | |
| decomplemented, cells and EVs-free human serum | 10 | HSDC10% |
| 50 | HSDC50% | |
| advanced RPMI 1640 | 10 | RPMI10% |
| 50 | RPMI50% | |
| cells and EVs-free secretome (100k fraction of secretome) | 10 | Secretome10% |
| 50 | Secretome50% |
a
All the excipients were dispersed in physiologic solution.