Table 2. Crystallization.
Method | Vapor diffusion, sitting drop |
Temperature (K) | 285 |
Protein concentration (mg ml−1) | 21.3 |
Buffer composition of protein solution | 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
Composition of reservoir solution | 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD, 20 mM sodium L-glutamate, 20 mM DL-alanine, 20 mM glycine, 20 mM DL-lysine·HCl, 20 mM DL-serine, 100 mM Bicine, 100 mM Trizma base pH 8.5 |
Volume ratio of drop | 1:1 |
Volume of reservoir (µl) | 80 |