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. 2023 Sep 20;79(Pt 10):257–266. doi: 10.1107/S2053230X23008002

Table 2. Crystallization.

Method Vapor diffusion, sitting drop
Temperature (K) 285
Protein concentration (mg ml−1) 21.3
Buffer composition of protein solution 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
Composition of reservoir solution 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD, 20 mM sodium L-glutamate, 20 mM DL-alanine, 20 mM glycine, 20 mM DL-lysine·HCl, 20 mM DL-serine, 100 mM Bicine, 100 mM Trizma base pH 8.5
Volume ratio of drop 1:1
Volume of reservoir (µl) 80