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. 2023 Jan 25;24(10):4419–4429. doi: 10.1021/acs.biomac.2c01095

Figure 3.

Figure 3

Investigation of biocompatibility of the EL-RGDS/PA membranes. Mouse-derived adipose stem cells (mADSCs) were cultured on the membranes. (A) Fluorescence microscopy images of mADSCS stained with DAPI after 24 h of cell culture. (B) Morphology study. mADSCs were stained with Phalloidin CruzFluor 647 and DAPI. Images indicate a spreading morphology and intercellular connections between mADSCS grown on both EL-RGDS/PAK2 and EL-RGDS/PAK3 membranes, in contrast to mADSCs grown on EL-RGDS/PAK2-K4, which were observed to be not spread and with minimal intercellular connections formed. (C) Cell adhesion density as calculated based on the adhesion study images stained with DAPI (A). (D) Proliferation studies. dsDNA content was quantified by PicoGreen assay. (E) Cell metabolic activity was assessed with Alamar Blue assay. Error bars represent ±SD. The experiments were performed in triplicates (n = 6).