PCR reaction master mix
| Reagent | Amount |
|---|---|
| DNA template | 2 μL |
| Q5 High-Fidelity 2× Master Mix | 25 μL |
| 10 μM Forward Primer (500 mM stock) | 2.5 μL |
| 10 μM Reverse Primer (500 mM stock) | 2.5 μL |
| Ambion Nuclease Free Water | 18 μL |
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