PCR cycling condition

Steps	Temperature	Time	Cycles
Initial Denaturation	98°C	30 s	1
Denaturation	98°C	5–10 s	35 cycles
Annealing	55°C–72°C	10–30 s
Extension	72°C	20–30 s/kb
FinalExtension	72°C	2 min	1
Hold	12°C	forever