Table 1.
The effect of dual ET antagonism on metabolic cage parameters in male and female rats fed a normal or high salt diet
| Diet | Normal salt (0.3% NaCl) | ||||
|---|---|---|---|---|---|
| Treatment | Control | Dual antagonist | ANOVA results | ||
| Sex | Male | Female | Male | Female | |
|
Food intake g/kg/day |
50.0 ± 0.84 | 47.5 ± 5.17 | 43.7 ± 2.29 | 59.8 ± 5.07* |
PInteraction = 0.0224 PSex = 0.0868 PTreatment = 0.4324 |
| Water intake ml/kg/day | 79.6 ± 5.51 | 88.3 ± 9.97 | 66.8 ± 5.29 | 109.1 ± 11.7* |
PInteraction = 0.0620 PSex = 0.0068 PTreatment = 0.6482 |
| Urine flow ml/kg/day | 26.7 ± 2.23 | 35.4 ± 8.46 | 25.1 ± 4.77 | 32.5 ± 8.67 |
PInteraction = 0.8527 PSex = 0.2446 PTreatment = 0.6587 |
| UNaV mg/kg/day | 50.9 ± 5.9 | 82.4 ± 7.2 | 69.7 ± 6.2 | 73.2 ± 14.3 |
PInteraction = 0.1360 PSex = 0.0658 PTreatment = 0.6008 |
|
UKV mg/kg/day |
247.0 ± 16.4 | 296.5 ± 52.4 | 233.8 ± 14.4 | 260.5 ± 32.1 |
PInteraction = 0.7302 PSex = 0.2539 PTreatment = 0.4579 |
|
UClV mg/kg/day |
138.8 ± 11.2 | 217.0 ± 40.4 | 163.8 ± 10.6 | 172.7 ± 25.0 |
PInteraction = 0.1780 PSex = 0.0943 PTreatment = 0.7022 |
| Diet | High salt (4% NaCl) | ||||
|---|---|---|---|---|---|
| Treatment | Control | Dual antagonist | ANOVA results | ||
| Sex | Male | Female | Male | Female | |
| Food intake g/kg/day | 52.3 ± 2.86 | 41.1 ± 1.97 | 32.5 ± 2.45† | 59.7 ± 4.27*†† |
PInteraction < 0.0001 PSex = 0.0138 PTreatment = 0.8375 |
| Water intake ml/kg/day | 98.1 ± 3.61 | 129.2 ± 18.20 | 110.4 ± 11.81 | 195.1 ± 15.96*†† |
PInteraction = 0.0831 PSex < 0.0001 PTreatment = 0.0030 |
| Urine flow ml/kg/day | 45.4 ± 2.66 | 71.9 ± 8.94 | 57.3 ± 6.91 | 122.1 ± 25.63* |
PInteraction = 0.1913 PSex = 0.0025 PTreatment = 0.0337 |
|
UNaV mg/kg/day |
225.6 ± 28.4 | 368.1 ± 56.5 | 334.6 ± 31.7 | 659.5 ± 91.2*†† |
PInteraction = 0.1272 PSex = 0.0005 PTreatment = 0.0020 |
|
UKV mg/kg/day |
357.0 ± 17.0 | 366.2 ± 38.3 | 261.7 ± 17.9 | 391.0 ± 33.6* |
PInteraction = 0.0443 PSex = 0.0222 PTreatment = 0.2253 |
|
UClV mg/kg/day |
410.4 ± 46.3 | 621.8 ± 87.2 | 523.8 ± 48.0 | 1001.9 ± 123.1*†† |
PInteraction = 0.1189 PSex = 0.0003 PTreatment = 0.0063 |
*P < 0.05 vs. corresponding male animals within the same treatment group. †P < 0.05 vs. corresponding male animals in the control group. ††P < 0.05 vs. corresponding female animals in the control group. N = 7 per group. Two-way ANOVA with post hoc Sidak’s test