Table 1.

Singlet fraction and mean F-score of each demultiplexing method for all batches in all data sets

Metric	BAL batch 1	BAL batch 2	BAL batch 3	OT batch 1	OT batch 2	CL
Genetic singlet fraction	21 851/24 091	41 816/48 841	50 496/62 306	11 296/12 510	8683/9547	40 118/45 977
F mean (hashedDrops)	0.930	0.847	0.785	0.679	0.813	0.864
F mean (demuxmix)	0.907	0.833	0.775	0.511	0.506	0.743
F mean (HTODemux)	0.892	0.794	0.742	0.620	0.654	0.737
F mean (GMM-Demux)	0.905	0.770	0.720	0.645	0.632	0.823
F mean (HashSolo)	0.920	0.783	0.737	0.606	0.688	0.806
F mean (deMULTIplex)	0.907	0.791	0.624	0.218	0.684	0.828
F mean (hashedDrops—default)	0.907	0.613	0.573	0.406	0.46	0.663
F mean (BFFraw)	0.879	0.539	0.466	0	0.623	0.791
F mean (BFFcluster)	0.935	0.119	0.188	0	0.408	0.874

The best-performing method for each batch is indicated in bold.