Skip to main content
. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Curr Protoc. 2023 Oct;3(10):e890. doi: 10.1002/cpz1.890

Figure 3. Representative data for arrestin-3 recruitment to β2AR in the NanoBiT assay.

Figure 3.

HEK arrestin-2/3 KO cells were transfected with SmBiT-arrestin-3 and β2AR-LgBiT. 48 h post-transfection, Nano-Glo reagent was added, and the luminescence was measured for 20 minutes. Isoproterenol was added after the 20-minute read of the background signal and the luminescence was measured for 60 minutes. (A) Real-time recruitment of arrestin-3 to β2AR. Arrestin-3 Δ7 was used as the negative control, as it does not bind to GPCR. (B) Dose-response curve that compares the recruitment of wildtype arrestin and the “enhanced” arrestin-3 mutant with C-terminus deleted to β2AR upon isoproterenol activation.