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(A) Schematic of arrestin translocation BRET assay. Arrestin is fused to Rluc8 and an unrelated plasma membrane marker is fused to the acceptor citrine. Activation of the unmodified receptor recruits arrestin to the plasma membrane, which increases BRET between the donor and acceptor molecules. (B) Representative concentration-response curves of an arrestin translocation BRET assay.
