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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Curr Protoc. 2023 Oct;3(10):e890. doi: 10.1002/cpz1.890

Figure 5. Comparison of direct and indirect NanoBiT assays in measurement of arrestin-3 recruitment to D2R.

Figure 5.

(A) Schematic of arrestin translocation NanoBiT assay. Arrestin is fused to SmBiT and an unrelated plasma membrane marker is fused to the LgBiT. Activation of the unmodified receptor recruits arrestin to the plasma membrane, which increases complementation. (B) HEK293 arrestin-2/3 KO cells were transfected with SmBiT-arrestin-3 and either a D2R fused with LgBiT (blue, direct assay) or a wildtype D2R with LgBiT anchored to the plasma membrane (brown, indirect assay).